Représentation des stats calculées par MetHis (50 000 SNP)

Librairies utilisées

library(ggplot2)
library(ggthemes)
library(lattice)
library(plot3D)
library(rriskDistributions)
library(stringr)
require(MASS)

## Loading required package: MASS

##Indicateur de temps

time.ini = Sys.time()

Fonctions utilisées

Fonction de lecture des stats résumées. La fonction est prévue pour lire les fichiers de stats résumées dans des répertoires de la forme “Ne100/simu_1” (en modifiant les valeurs de Ne et de simulations). Elle renvoie les résultats sous forme de data frame en ajoutant aux statistiques résumées les colonnes correspondant à l’effectif efficace et la simulation.

#Data frame construction
data.frame.stat = function(seq_ne, max_gen, max_simu){

  #Créer matrice vide qui contiendra les stats
  mat_stat = matrix(data = NA, nrow = length(seq_ne)*max_gen*max_simu, ncol = 64)
  mat_stat = as.data.frame(mat_stat)
  #Compteur pour indiquer les lignes à remplir
  cpt = 0

  for (ne in seq_ne) {
    if (dir.exists(str_c("Ne", toString(ne), "/"))) {
      dir_ne = str_c("Ne", toString(ne), "/") #Répertoire de taille effective
      row_min = cpt*max_gen*max_simu+1 #Ligne min. où on écrit les stats pour le Ne donné
      row_max = (cpt+1)*max_gen*max_simu         #Ligne max. où on écrit les stats  
      mat_stat[row_min:row_max,1] = toString(ne) #Ecriture du Ne correspondant pour les stats qui seront écrites
      cpt = cpt+1                             #Incrémentation du compteur
  
      for (nb_simu in 1:max_simu) {
        dir_simu = str_c("simu_", toString(nb_simu), "/")
        row_min_simu = (nb_simu-1)*max_gen + row_min
        row_max_simu = nb_simu*max_gen + row_min -1
        mat_stat[row_min_simu:row_max_simu,2] = nb_simu
        file_path = str_c(dir_ne, dir_simu, "final_sumstats.txt")
        file_stat = read.table(file_path, header = TRUE) #Lecture fichier stat résumées
        #Suppression 1ère colonne, contenant uniquement chiffres pour tri en bash. 
        mat_stat[row_min_simu:row_max_simu,3:63] = file_stat
        #Ajout de la contribution s1,0 initiale pour pouvoir calculer les delta de proportion d'admixture ultérieurement
        file_path_s1.0 = str_c(dir_ne, dir_simu, str_c("simu_",nb_simu,".txt") )
        file_stat_s1.0 = read.table(file_path_s1.0, header = TRUE)
        mat_stat[row_min_simu:row_max_simu,64] = file_stat_s1.0$s1.0[1]
      }
      #print(dim(mat_stat))
    }
  }
  

  #Attribution nom colonnes selon appelation stat par MetHis
  colnames(mat_stat) = c("Ne", "simu", names(file_stat), "s1.0")#,"cpt")#, "delta.adm.props")
  #Passage des Ne en facteur
  mat_stat$Ne = factor(as.factor(mat_stat$Ne), levels = as.character(seq_ne))
  #Passage simulations en entier
  mat_stat$simu = as.integer(mat_stat$simu)
  #Passage générations en entier
  mat_stat$Generation = as.integer(mat_stat$Generation)
  #Passage stats en double
  for (numcol in 4:ncol(mat_stat)) {
    mat_stat[,numcol] = as.double(mat_stat[,numcol])
  }
  
  # print(dim(mat_stat))
  
  return(mat_stat)
}

Calcul du delta des moyennes de proportion d’admixture par rapport à la moyenne attendue

var.adm = function(s1, gen){
  return( (s1*(1-s1))/(2**gen) )
}

delta.adm.props = function(mat){
  mat$delta.mean.adm.props = NA
  mat$delta.var.adm.props = NA
  for (i in 1:nrow(mat)) {
    ref_adm = mat$s1.0[i]
    mat$delta.mean.adm.props[i] = mat$mean.adm.props[i] - ref_adm
    ref_var = var.adm(s1 = ref_adm, gen = mat$Generation[i])
    mat$delta.var.adm.props[i] = mat$var.adm.props[i] - ref_var
  }
  return(mat)
}

Dans le cas où l’on travaille sur de très nombreuses simulations correspondant à un effectif efficace, cette fonction permet de calculer les statistiques moyennées pour les valeurs de Ne étudiées.

#Passage en moyenne
data.frame.mean = function(df_stat, max_gen, seq_ne){
  
  lrow = length(seq_ne)
  df_mean = matrix(data = NA, nrow = lrow*max_gen, ncol = 62 )
  df_mean = as.data.frame(df_mean)
  cpt = 0

  for (ne in seq_ne) {
    df_tmp = df_stat[which(df_stat$Ne == ne),]
    
    row_min = cpt*max_gen+1
    row_max = (cpt+1)*max_gen
    df_mean[row_min:row_max, 1] = toString(ne) 
    cpt = cpt+1
    
    for (gen in 0:(max_gen-1) ) {
      df_mean[row_min+gen, 2] = gen
      vec_tmp = apply(df_tmp[which(df_tmp$Generation == gen), 4:63], 2, mean)
      df_mean[row_min+gen, 3:62] = vec_tmp
    }
  }
  
  colnames(df_mean) = colnames(df_stat)[-2]
  df_mean$Ne = factor(as.factor(df_mean$Ne), levels = seq_ne )
  return(df_mean)
}

Fonction d’extraction d’une sous-matrice à partir d’une matrice principale en fonction de valeurs choisies de Ne devant être présentes dans la matrice initiale.

extract_sub_mat = function(all_mat, list_ne){
  all_rows = c()
  for (ne in as.character(list_ne)) {
    all_rows = append(all_rows, which(all_mat[,1] == ne))
  }
  return(all_mat[all_rows,])
}

Fonction d’affichage des graphiques en 2D, avec un background blanc et les lignes horizontales de quadrillage affichées. Cette fonction permet d’afficher les lignes reliant les points ou les lignes de régression. Il est possible de passer en paramètres la variable selon laquelle les points seront colorés, les groupes selon lesquels les points sont reliés, l’affichage ou non de la légende (l’affichage se fait par défaut), la taille des points et le type de ligne utilisé.

#Plot function
#Affichage d'une stat au cours des générations
plot_stat_gen = function(df, gen, stat, group_col = c(), color_col, titre, ligne = TRUE, legd = TRUE,
                         size_point = 0.1, line_t = "solid"){
  p = ggplot(df, aes(x = gen, y = stat,
                     group = group_col, color = color_col)) + ggtitle(titre)
  
  if (ligne) {
    p = p + geom_point(size = size_point, show.legend = legd)+
      geom_line(aes(linetype = line_t), show.legend = legd)
  }else{
    #smooth de la courbe pour obtenir régression et tempérer variabilité due au TCL
    p = p + geom_point(size = size_point, show.legend = legd)+ geom_smooth(show.legend = legd)
  }
  p = p + theme_classic()
  p = p + theme(panel.grid.major.y = element_line(size = 0.5,
                                                  linetype = 'solid',
                                                  colour = "#BABABA"),
                panel.grid.minor.y = element_line(size = 0.25,
                                                  linetype = 'solid',
                                                  colour = "#CECECE"))
  
  return(p)
}

Cette fonction d’affichage de graphique reprend la précédente, en affichant uniquement les courbes de régression sans les points.

#Plot function
#Affichage d'une stat au cours des générations
plot_without_point = function(df, gen, stat, color_col, titre, legd = TRUE){
  p = ggplot(df, aes(x = gen, y = stat, color = color_col)) + ggtitle(titre)
  
    #smooth de la courbe pour obtenir régression et tempérer variabilité due au TCL
  p = p + geom_smooth(show.legend = legd)
  p = p + theme_classic()
  p = p + theme(panel.grid.major.y = element_line(size = 0.5,
                                                  linetype = 'solid',
                                                  colour = "#BABABA"),
                panel.grid.minor.y = element_line(size = 0.25,
                                                  linetype = 'solid',
                                                  colour = "#CECECE"))
  #print(p)
  return(p)
}

Cette fonction permet de rajouter à un plot existant : - des lignes verticales (pour les goulots d’étranglement par exemple) - un titre aux légendes de couleurs et de types de ligne - de modifier les types de lignes utilisés

improve_plot_bottle = function(p, vec_abline, lab_col, lab_line, vec_linetype){
  for (rg_abl in 1:length(vec_abline)) {
    p = p + geom_vline(xintercept = vec_abline[rg_abl], size = 0.4, color = "orange", linetype = "solid")
  }
  p = p + labs(color = lab_col, linetype = lab_line) + scale_linetype_manual(values=vec_linetype)
  return(p)
}

Fonction pour une représentation graphique à l’aide d’un gif, pour suivre l’évolution de la distribution des proportions d’admixture dans la population.

hist.gif.props.adm = function(mat, select_stat, select_seq, title, title_leg,
                              seq_gen = c(seq(1,10,1), seq(11,101,10)),
                              vec_col = c("#A5260A", "#F6DC12", "#87E990", "#FFE4C4",
                                          "#E67E30", "#79F8F8", "#A10684", "#8B6C42")){
  
  png(file="example%03d.png", width=600, heigh=400)
  for (line in seq_gen) {
    cpt_col = 1
    for (var in select_seq) {
      rg_select = which(colnames(mat) == select_stat)
      mat_tmp = mat[which(mat[,rg_select] == var),]
      vec_y = c(mat_tmp$perc0.adm.props[line],
                mat_tmp$perc10.adm.props[line],
                mat_tmp$perc20.adm.props[line],
                mat_tmp$perc30.adm.props[line],
                mat_tmp$perc40.adm.props[line],
                mat_tmp$perc50.adm.props[line],
                mat_tmp$perc60.adm.props[line],
                mat_tmp$perc70.adm.props[line],
                mat_tmp$perc80.adm.props[line],
                mat_tmp$perc90.adm.props[line],
                mat_tmp$perc100.adm.props[line])

      if (var == select_seq[1]) {
        hist(vec_y, breaks = vec_y, main = line-1, col = vec_col[cpt_col], xlim = c(-0.2,1.2))
        cpt_col = cpt_col+1
      }else{
        hist(vec_y, breaks = vec_y, add = TRUE, col = vec_col[cpt_col])
        cpt_col = cpt_col+1
      }
    }
    legend("topleft", title=title_leg,legend = select_seq,
           fill = vec_col[1:cpt_col], cex=0.8) 
  }

  dev.off()
  system(str_c("convert -delay 120 *.png ",title,".gif"))
  file.remove(list.files(pattern=".png"))

}

###Enregistrement des fonctions

print(difftime(Sys.time(), time.ini))

## Time difference of 0.07715964 secs

Population constante de tailles initiales différentes

Calcul des matrices de stats pour de populations constantes de tailles initiales différentes.

seq_1024_16384 = 2**seq(10,14,1)
seq_50_1000 = seq(50,1000,1)
seq_tot = sort(c(seq_50_1000, seq_1024_16384))

# mat tot cstt
mat_tot = data.frame.stat(seq_tot, 101, 1) 
# Ne de 50 à 200 par pas de 10 puis de 200 à 1000 par pas de 100 (mat1 cstt)
mat_50_1000 = extract_sub_mat(mat_tot, c(seq(50,200,10), seq(200,1000,100)))
# Ne de 1024 à 16384 avec un pas de x2 (mat2 cstt)
mat_1024_16384 = extract_sub_mat(mat_tot, 2**seq(6,14,1))
# Ne de 64 à 16384 avec un pas de x2 + Ne de 100 et de 1000 (mat3 cstt)
mat_64_100_16384 = extract_sub_mat(mat_tot, c(2**seq(6,14,1), 100, 1000) )

Graphiques obtenus : affichage de l’évolution d’une statistique donnée en fonction des générations colorée selon la Ne initiale.

#####Hétérozygotie moyenne

# Affichage de l'hétérozygotie pour la matrice totale (mat tot cstt)
p = plot_stat_gen(mat_tot, mat_tot$Generation, 
                  mat_tot$mean.het.adm, mat_tot$Ne, mat_tot$Ne,ligne = T,
                  "Moyenne He en fonction des générations\n selon différentes Ne initiales",
                  legd = FALSE)
p = p+geom_hline(yintercept = mat_50_1000$mean.het.s1[1], color = "red")
p = p+geom_hline(yintercept = mat_50_1000$mean.het.s2[1], color = "black")
print(p)

# Affichage de l'hétérozygotie pour mat1 cstt
p = plot_stat_gen(mat_50_1000, mat_50_1000$Generation, 
                      mat_50_1000$mean.het.adm, mat_50_1000$Ne, mat_50_1000$Ne,ligne = T,
                      "Moyenne He en fonction des générations\nselon différentes Ne initiales",
                      legd = TRUE)
p = p+geom_hline(yintercept = mat_50_1000$mean.het.s1[1], color = "red")
p = p+geom_hline(yintercept = mat_50_1000$mean.het.s2[1], color = "black")
print(p)

#####Fst s1 adm

plot_stat_gen(mat_tot, mat_tot$Generation, 
              mat_tot$Fst.s1.adm, mat_tot$Ne,mat_tot$Ne,
              "Fst s1 adm en fonction des générations\n selon différentes Ne initiales",
              legd = FALSE)

plot_stat_gen(mat_50_1000, mat_50_1000$Generation, 
              mat_50_1000$Fst.s1.adm, mat_50_1000$Ne,mat_50_1000$Ne,
              "Fst s1 adm en fonction des générations\n selon différentes Ne initiales")

plot_stat_gen(mat_1024_16384, mat_1024_16384$Generation, 
              mat_1024_16384$Fst.s1.adm, mat_1024_16384$Ne,mat_1024_16384$Ne,ligne = T,
              "Fst s1 adm en fonction des générations\n selon différentes Ne initiales")

Fonction d’écriture de plot dans des fichiers, en choisissant la statistique enregistrée.

write_cstt_plot = function(name_stat, mat, name_file,min_y, max_y){
  num_col = which(colnames(mat) == name_stat)
  p = plot_stat_gen(mat, mat$Generation, 
                mat[,num_col], mat$Ne, mat$Ne,ligne = T,
                str_c(name_stat, " en fonction des générations\n selon différentes Ne initiales"))
  p = p+ylim(min_y, max_y)+ labs(color = "Ne") + guides(linetype = FALSE)
  png(filename = str_c("../../../Images/", name_file, ".png"), width = 1000, height = 800)
  print(p)
  dev.off()
  ggsave(filename =  str_c("../../../Images/", name_file, ".eps"), plot = p)
}

Ajout delta mean et var des proportions d’admixture pour les effectifs efficaces constants.

mat_64_100_16384 = delta.adm.props(mat_64_100_16384)

Écriture de différents graphiques pour différentes statistiques choisies. La matrice choisie est la mat3 cstt pour des raisons de lisibilité. Cette matrice permet également d’observer l’évolution des statistiques choisies pour des Ne = 100 et Ne = 1000, valeurs qui seront reprises dans d’autres scénarios ultérieurement.^

write_cstt_plot("mean.het.adm", mat_64_100_16384, "moyenne_heterozygotie/moy_He_ne_cst", 0.01, 0.062)

## Saving 7 x 5 in image

write_cstt_plot("Fst.s1.adm", mat_64_100_16384, "Fsts1adm/fst_s1_adm_ne_cst", -0.01, 0.5)

## Saving 7 x 5 in image

write_cstt_plot("Fst.s2.adm", mat_64_100_16384, "Fsts2adm/fst_s2_adm_ne_cst", -0.01, 0.5)

## Saving 7 x 5 in image

write_cstt_plot("mean.F.adm", mat_64_100_16384, "Fadm/f_adm_ne_cst", -0.03, 0.03)

## Warning: Removed 6 rows containing missing values (geom_point).

## Warning: Removed 1 row(s) containing missing values (geom_path).

## Saving 7 x 5 in image

## Warning: Removed 6 rows containing missing values (geom_point).

## Warning: Removed 1 row(s) containing missing values (geom_path).

write_cstt_plot("delta.mean.adm.props", mat_64_100_16384, "delta_mean_adm/delta_mean_ne_cstt", -0.25, 0.25)

## Saving 7 x 5 in image

write_cstt_plot("delta.var.adm.props", mat_64_100_16384, "delta_var_adm/delta_var_ne_cstt", -0.2, 0.05)

## Saving 7 x 5 in image

setwd("../../../Images/")

hist.gif.props.adm(mat = mat_64_100_16384, select_stat = "Ne", select_seq = c(64,100,256,2048),
                   title = "adm.props/hist_cstt", title_leg = "Ne")

##  [1] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
## [16] TRUE TRUE TRUE TRUE TRUE

###Populations à Ne constants

print(difftime(Sys.time(), time.ini))

## Time difference of 2.11358 mins

Populations croissantes

Réutilisation de la fonction de lecture de stats résumées pour des populations croissantes. Celles-ci sont enregistrées dans des répertoires de la forme “Ne100-XXX/Ne100-1000/simu_1” Fonction de lecture des stats résumées. La fonction est prévue pour lire les fichiers de stats résumées dans des répertoires de la forme “NeXXX/simu_XXX” (avec XXX remplacés par les valeurs de Ne et de simulations). Elle renvoie les résultats sous forme de data frame en ajoutant aux statistiques résumées les colonnes correspondant à l’effectif efficace et la simulation.

data.frame.ne.gen = function(seq_ne){
  for (ne in seq_ne) {
    if (dir.exists(str_c("Ne", ne))) {
      dir_ne = str_c("Ne", ne, "/simu_1/")
      setwd(dir_ne)
      file_tmp = read.table("simu_1.par", header = TRUE)
      
      if (ne == seq_ne[1]) {
        mat_ne_inc = as.data.frame(file_tmp[,2])
      }else{
        mat_ne_inc = cbind(mat_ne_inc, file_tmp[,2])
      }
      setwd("../../")
    }
  }
  return(mat_ne_inc)
}

data.frame.increase = function(seq_ne_ini, seq_combi){
  mat_inc = c()
  for (ne_ini in seq_ne_ini) {
    if (dir.exists(str_c("Ne", ne_ini, "-XXX/"))) {
      dir_ne = str_c("Ne", ne_ini, "-XXX/")
      setwd(dir_ne)
      motif_detect = str_c("^",ne_ini,"-")
      vec_ne_tmp = seq_combi[which(str_detect(seq_combi, motif_detect))]
      mat_tmp = data.frame.stat(seq_ne = vec_ne_tmp, max_gen = 101, max_simu = 1)
      mat_ne = data.frame.ne.gen(seq_ne = vec_ne_tmp)
      # print(mat_ne)
      vec_tmp = as.integer(unlist(str_split(mat_tmp$Ne, "-")))
      mat_tmp$ne_gen = unlist(mat_ne, use.names = F)
      # print(length(vec_tmp))
      mat_tmp$n0 = vec_tmp[seq(1,length(vec_tmp),2)]
      mat_tmp$nf = vec_tmp[seq(2,length(vec_tmp),2)]
      if (ne_ini == seq_ne_ini[1]) {
        mat_inc = mat_tmp
      }else{
        mat_inc = rbind(mat_inc, mat_tmp)
      }
      
      setwd("../")
      #print(dim(mat_pop_inc))
    }
  }
  return(na.omit(mat_inc))
}

# mat_pop_nu = data.frame(mat_pop_nu,
#                         ne_gen = unlist(mat_ne_inc , use.names = F),
#                         n0 = rep(NA, nrow(mat_pop_nu)),
#                         nf = rep(NA, nrow(mat_pop_nu)) )
# 
# for (i in 1:nrow(mat_pop_nu)) {
#   vec_tmp = as.integer(unlist(str_split(mat_pop_nu$Ne, "-")))
#   mat_pop_nu$n0[i] = vec_tmp[1]
#   mat_pop_nu$nf[i] = vec_tmp[2]
# }
# 
# mat_ne_inc = data.frame()
# cpt = 1

Lecture des data frame pour les combinaison de Ne entre ne0 (ne_ini) et nef (ne_fin). On extrait ensuite les stats pour lesquelles on a un nef de 10000.

setwd("../new_methis_pop_increase_50000_snp/")
seq_ne_ini = seq(50,100,2)
seq_ne_fin = c(seq(100, 10000, 200), 10000)
seq_combi = as.data.frame(outer(seq_ne_ini, seq_ne_fin, FUN="paste", sep="-"))
seq_combi = as.data.frame.vector(unlist(seq_combi))[[1]]

mat_pop_inc = data.frame.increase(seq_ne_ini, seq_combi)


mat_end_10000 = extract_sub_mat(mat_pop_inc,
                                as.data.frame(outer(seq(50,100,4),
                                                    c("10000"),
                                                    FUN="paste",
                                                    sep="-"))[,1])

plot_stat_gen(df = mat_pop_inc, gen = mat_pop_inc$Generation,
              stat = mat_pop_inc$f3, group_col = mat_pop_inc$Ne,color_col = mat_pop_inc$Ne,
              titre = "Stat en fonction des générations\n selon différentes Ne initiales",
              legd = FALSE)

plot_stat_gen(mat_pop_inc, mat_pop_inc$Generation, 
              mat_pop_inc$mean.het.adm, mat_pop_inc$Ne,mat_pop_inc$Ne,
              "Stat en fonction des générations\n selon différentes Ne initiales",
              legd = FALSE)

plot_stat_gen(mat_end_10000, mat_end_10000$Generation, 
              mat_end_10000$mean.het.adm, mat_end_10000$Ne,mat_end_10000$Ne,
              "Moyenne hétérozygotie en fonction des générations\n selon différentes Ne initiales",
              legd = TRUE)

plot_stat_gen(mat_end_10000, mat_end_10000$Generation, 
              mat_end_10000$f3, mat_end_10000$Ne,mat_end_10000$Ne,
              "F3 en fonction des générations\n selon différentes Ne initiales",
              legd = TRUE)

###Populations croissantes à U aléatoire dans la loi uniforme [0,0.5]

print(difftime(Sys.time(), time.ini))

## Time difference of 2.762601 mins

Variation U

Le U déterminant l’inflexion de la courbe de croissance est ici connu et fixé avant la simulation.

data.frame.increase.nu = function(seq_ne_ini, seq_combi, seq_nu){
  for (nu in seq_nu) {
    if(dir.exists(str_c("Nu", nu ))){
      dir_nu = str_c("Nu", nu, "/")
      setwd(dir_nu)
      
      mat_tmp = data.frame.increase(seq_ne_ini, seq_combi)
      
      if (nu == seq_nu[1]) {
        mat_pop = data.frame(mat_tmp, U = nu)
      }else{
        mat_tmp = data.frame(mat_tmp, U = nu)
        mat_pop = rbind(mat_pop, mat_tmp)
      }
      setwd("../")
    }
  }
  mat_pop$U = as.factor(mat_pop$U)
  
  return(mat_pop)

}

setwd("../new_methis_pop_increase_50000_snp_Nu_var/")

#Détermination des paramètres initiaux
seq_nu = sprintf("%.2f", seq(0.05, 0.45, 0.05))
seq_ne_ini = seq(50,100,10)
seq_ne_fin = seq(100, 10000, 200)
seq_combi = as.data.frame(outer(seq_ne_ini, seq_ne_fin, FUN="paste", sep="-"))
seq_combi = as.data.frame.vector(unlist(seq_combi))[[1]]


mat_pop_nu = data.frame.increase.nu(seq_ne_ini = seq_ne_ini, seq_combi = seq_combi, seq_nu = seq_nu)

plot_without_point(mat_pop_nu, mat_pop_nu$Generation, 
                   mat_pop_nu$mean.het.adm, mat_pop_nu$Ne,
                   "Moyenne hétérozygotie en fonction des générations\n selon différentes Ne initiales\n et couleurs selon U",
                   legd = FALSE)

## `geom_smooth()` using method = 'loess' and formula 'y ~ x'

plot_without_point(mat_pop_nu, mat_pop_nu$Generation, 
                   mat_pop_nu$mean.het.adm, mat_pop_nu$U,
                   "Moyenne hétérozygotie en fonction des générations\n selon différentes Ne initiales\n et couleurs selon nu",
                   legd = TRUE)

## `geom_smooth()` using method = 'gam' and formula 'y ~ s(x, bs = "cs")'

# plot_stat_gen(mat_pop_nu, mat_pop_nu$Generation,
#              mat_pop_nu$mean.het.adm, mat_pop_nu$Ne,
#              "Stat en fonction des générations\n selon différentes Ne initiales",
#              TRUE, legd = FALSE)
# plot_stat_gen(mat_pop_nu, mat_pop_nu$Generation,
#              mat_pop_nu$mean.het.adm, mat_pop_nu$Nu,
#              "Stat en fonction des générations\n selon différentes Ne initiales",
#              TRUE, legd = FALSE)

Affichage de l’hétérozygotie pour les différentes valeurs de Nu avec N0 entre 50 et 100 (pas de 10) et Nf entre 100 et 10 000 (pas de 200).

for (nu in seq_nu) {
  
  mat_pop_nu_tmp = mat_pop_nu[which(mat_pop_nu$U == nu),]
  
  p = plot_stat_gen(mat_pop_nu_tmp, mat_pop_nu_tmp$Generation,
                        mat_pop_nu_tmp$mean.het.adm, mat_pop_nu_tmp$Ne,
                        mat_pop_nu_tmp$Ne,
                        str_c("U = ",nu), TRUE, legd = FALSE)
  p = p+geom_hline(yintercept = mat_pop_nu_tmp$mean.het.s1[1],
                           color = "red")
  p = p+geom_hline(yintercept = mat_pop_nu_tmp$mean.het.s2[1],
                           color = "black")
  print(p)

}

remove(mat_pop_nu_tmp)

Affichage en surface de l’hétérozygotie à la génération 100 en fonction de Ne0 et Nef selon différents U.

for (nu in seq_nu) {
  
  mat_pop_nu_tmp = mat_pop_nu[which(mat_pop_nu$U == nu),]
  
  list_ne = mat_pop_nu_tmp$Ne[which(mat_pop_nu_tmp$Generation == 100)]
  list_ne = as.integer(unlist(str_split(list_ne, "-")))
  list_ne0 = list_ne[seq(1, length(list_ne), 2)]
  list_nef = list_ne[seq(2, length(list_ne), 2)]
  mat_pop_nu_tmp = mat_pop_nu_tmp[which(mat_pop_nu_tmp$Generation == 100),]
  
  print(wireframe(mat_pop_nu_tmp$mean.het.adm ~ list_ne0 * list_nef, data = mat_pop_nu_tmp,
                  drape = TRUE, zlab = "He", xlab = "Ne0", ylab = "Nef",
                  screen = list(z = 40, x = -60),
                  scales = list(z.ticks=5, arrows=F, col="black", font=1, tck=0.8),
                  main = str_c("He moyenne à la génération 100 en fonction de Ne0 et Nef\n U = ", nu)  ))
  
}

remove(mat_pop_nu_tmp)

for (rg_nu in seq_nu) {
  #rg_nu
  df_tmp = mat_pop_nu[which(mat_pop_nu$U == rg_nu),]
  
  scatter3D(x = df_tmp$Generation, y = df_tmp$ne_gen, z = df_tmp$mean.het.adm,
            bty = "b2", pch = 16, ticktype = "detailed", main = rg_nu,
            theta = 210, phi = 20, type = "s",cex = 0.2, cex.lab = 0.6, cex.axis = 0.5,
            xlab = "Gen", ylab = "Ne", zlab = "Stat")
  # print(wireframe(mean.het.adm ~ Generation * ne_gen,
  #                 data = df_tmp,
  #                 drape = TRUE, zlab = "Htz", xlab = "generation", ylab = "Ne gen",
  #                 screen = list(z = 40, x = -60),
  #                 scales = list(z.ticks=5, arrows=F, col="black", font=1, tck=0.8),
  #                 main = rg_nu))
}

rm(df_tmp)

scatter3D(x = mat_pop_nu$Generation, y = mat_pop_nu$ne_gen,
          z = as.numeric(as.character(mat_pop_nu$U)),
          bty = "b2", pch = 16, ticktype = "detailed",
          theta = 210, phi = 50, type = "s", cex.lab = 0.6, cex.axis = 0.5,
          xlab = "Gen", ylab = "Ne", zlab = "Nu")

setwd("../new_methis_pop_increase_50000_snp_Nu_var/")
seq_nu_bis = sprintf("%.2f", c(0.01, 0.25, 0.49))
seq_ne_ini = c(100)
seq_ne_fin = c(200, 400, seq(1000, 10000, 1000))
seq_combi = as.data.frame(outer(seq_ne_ini, seq_ne_fin, FUN="paste", sep="-"))
seq_combi = as.data.frame.vector(unlist(seq_combi))[[1]]

mat_pop_nu_reduce = data.frame.increase.nu(seq_ne_ini, seq_combi, seq_nu_bis)

mat_pop_nu_reduce_gen_100 = mat_pop_nu_reduce[which(mat_pop_nu_reduce$Generation == 100),]
mat_pop_nu_reduce$log_mean = log(mat_pop_nu_reduce$mean.het.adm)
scatter3D(x = mat_pop_nu_reduce$Generation, y = mat_pop_nu_reduce$nf,
          z = mat_pop_nu_reduce$mean.het.adm, colvar = as.numeric(mat_pop_nu_reduce$U),
          col = c("#1B9E77", "#D95F02", "#7570B3"),
          colkey = list(at = c(1.3, 2, 2.7),
                        addlines = TRUE, length = 1, width = 0.5,
                        labels = levels(as.factor(mat_pop_nu_reduce$U))),
          ticktype = "detailed",bty = "g",pch = 16,
          theta = 115, phi = 0, type = "s", cex = 0.5, cex.lab = 0.6, cex.axis = 0.5,
          xlab = "generation", ylab = "Nf", zlab = "stat", clab = "U")

  # scatter3D(x = df_tmp$Generation, y = vec_tot, z = df_tmp$mean.het.adm,
  #           bty = "b2", pch = 16, ticktype = "detailed", main = seq_nu[rg_nu],
  #           theta = 210, phi = 20, type = "s", cex.lab = 0.6, cex.axis = 0.5,
  #           xlab = "Gen", ylab = "Ne", zlab = "Stat")

setwd("../new_methis_pop_increase_50000_snp_Nu_var/")

seq_ne_ini3 = seq(100,100,0)
seq_multi3 = c(2,4,10,100)
seq_ne_fin3 = seq_multi3*seq_ne_ini3
seq_combi3 = as.data.frame(outer(seq_ne_ini3, seq_ne_fin3, FUN="paste", sep="-"))
seq_combi3 = as.data.frame.vector(unlist(seq_combi3))[[1]]

mat_pop_nu_reduce_3 = data.frame.increase.nu(seq_ne_ini3, seq_combi3, seq_nu_bis)

write_increase_plot = function(name_stat, mat, name_file, min_y, max_y){
  num_col = which(colnames(mat) == name_stat)
  p = plot_stat_gen(df = mat, gen = mat$Generation, 
                    stat = mat[,num_col], group_col = c(),
                    color_col = mat$Ne, line_t = mat$U,
                    titre = str_c(name_stat," en fonction des générations\n selon différentes croissances de populations"),
                    legd = TRUE)
  
  p = improve_plot_bottle(p =p, vec_abline = c(), lab_col = "ne0-nef",
                       lab_line = "U", vec_linetype = c("dotted", "longdash", "solid"))
  p = p + ylim(min_y, max_y)
  png(filename = str_c("../../../Images/", name_file,".png"), width = 1000, height = 800)
  print(p)
  dev.off()
  ggsave(filename =  str_c("../../../Images/", name_file, ".eps"), plot = p)
}

Ajout delta mean et var des proportions d’admixture pour les populations croissantes.

mat_pop_nu_reduce_3 = delta.adm.props(mat_pop_nu_reduce_3)

write_increase_plot("mean.het.adm", mat_pop_nu_reduce_3, "moyenne_heterozygotie/moy_He_pop_inc_nu_var",0.01,0.062)

## Saving 7 x 5 in image

write_increase_plot("Fst.s1.adm", mat_pop_nu_reduce_3, "Fsts1adm/fst_s1_adm_pop_inc_nu_var", 0, 0.5)

## Warning: Removed 2 rows containing missing values (geom_point).

## Warning: Removed 2 row(s) containing missing values (geom_path).

## Saving 7 x 5 in image

## Warning: Removed 2 rows containing missing values (geom_point).

## Warning: Removed 2 row(s) containing missing values (geom_path).

write_increase_plot("Fst.s2.adm", mat_pop_nu_reduce_3, "Fsts2adm/fst_s2_adm_pop_inc_nu_var", 0, 0.5)

## Saving 7 x 5 in image

write_increase_plot("mean.F.adm", mat_pop_nu_reduce_3, "Fadm/f_adm_pop_inc_nu_var", -0.03, 0.03)

## Warning: Removed 1 rows containing missing values (geom_point).

## Saving 7 x 5 in image

## Warning: Removed 1 rows containing missing values (geom_point).

write_increase_plot("delta.mean.adm.props", mat_pop_nu_reduce_3, "delta_mean_adm/delta_mean_pop_inc_nu_va", -0.25, 0.25)

## Saving 7 x 5 in image

write_increase_plot("delta.var.adm.props", mat_pop_nu_reduce_3, "delta_var_adm/delta_var_pop_inc_nu_va", -0.2, 0.05)

## Saving 7 x 5 in image

setwd("../../../Images/")

mat_pop_nu_reduce_100_10000 = mat_pop_nu_reduce_3[which(mat_pop_nu_reduce_3$Ne == "100-10000"),]

hist.gif.props.adm(mat = mat_pop_nu_reduce_100_10000, select_stat = "U", select_seq = seq_nu_bis,
                   title = "adm.props/hist_inc", title_leg = "U")

##  [1] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
## [16] TRUE TRUE TRUE TRUE TRUE

###Populations croissantes avec Nu variable et fixé

print(difftime(Sys.time(), time.ini))

## Time difference of 4.322928 mins

Bottleneck

data.frame.bottleneck = function(lst_ne0, lst_nef, lst_alpha, lst_nu, lst_bottle){
  lst_combi = as.data.frame(outer(lst_ne0, lst_nef, FUN="paste", sep="-"))
  lst_combi = as.data.frame.vector(unlist(lst_combi))[[1]]
  cpt = 0
  for (alpha in lst_alpha) {
    for (nu in lst_nu) {
      for (bottle in lst_bottle) {
        change_dir = str_c("alpha", alpha, "/Nu", nu, "/bottle",bottle,"/")
        # print(change_dir)
        if(dir.exists(change_dir)){
          # print(getwd())
          setwd(change_dir)
          
          mat_tmp = data.frame.increase(lst_ne0, lst_combi)
          if (!is.null(nrow(mat_tmp))) {
            col_alpha = rep(alpha, nrow(mat_tmp))
            col_nu = rep(nu, nrow(mat_tmp))
            col_bottle = rep(bottle, nrow(mat_tmp))
            col_bott_u = rep(str_c(bottle, "/", nu), nrow(mat_tmp))
            # col_bott_u = as.numeric(rep(bottle, nrow(mat_tmp)))/as.numeric(rep(nu, nrow(mat_tmp)))
            # col_bott_u = ceiling(col_bott_u/1)*1
            col_alpha_u = rep(str_c(nu, "/", alpha), nrow(mat_tmp))
            # col_alpha_u = as.numeric(rep(nu, nrow(mat_tmp)))/as.numeric(rep(alpha, nrow(mat_tmp)))
            # col_alpha_u = ceiling(col_alpha_u/0.001)*0.001
            mat_tmp = data.frame(mat_tmp, alpha = col_alpha,
                                 U = col_nu, time_botl = col_bottle,
                                 bott_u = col_bott_u, alpha_u = col_alpha_u)
            cpt = cpt+1
          }
          # print(dim(mat_tmp))
          if (alpha==lst_alpha[1] & nu==lst_nu[1] & bottle==lst_bottle[1]) {
            mat_bot = mat_tmp
          }else{
            mat_bot = rbind(mat_bot, mat_tmp)
          }
          setwd("../../../")
        }
      }
    }
  }
  if(!is.null(mat_bot)){
    mat_bot$alpha = as.factor(mat_bot$alpha)
    mat_bot$U = as.factor(mat_bot$U)
    mat_bot$time_botl = as.factor(mat_bot$time_botl)
    mat_bot$bott_u = as.factor(mat_bot$bott_u)
    mat_bot$alpha_u = as.factor(mat_bot$alpha_u)
  }
  return(mat_bot)
}

Nu fixé, alpha variable, N0 fixé, Nf fixé

####N0 = Nf = 1000

setwd("../new_methis_bottleneck_50000_snp/")

mat_bottle = data.frame.bottleneck(lst_ne0 = c(1000), lst_nef = c(1000), 
                                   lst_alpha = c(0.1, 0.5, 0.9),
                                   lst_nu = c(0.01, 0.25, 0.49),
                                   lst_bottle = seq(10, 90, 10))


mat_bottle_time_50 = mat_bottle[which(mat_bottle$time_botl == 50),]
mat_bottle_time_20 = mat_bottle[which(mat_bottle$time_botl == 20),]
mat_bottle_time_80 = mat_bottle[which(mat_bottle$time_botl == 80),]

boucle_plot_bottle = function(mat, vec_stat, name_stat, vec_bott,size_pop = 1000){
  for (bott in vec_bott) {
    new_mat = mat[which(mat$time_botl == bott),]
    new_vec = vec_stat[which(mat$time_botl == bott)]
    
    p = plot_stat_gen(new_mat, new_mat$Generation, 
                      new_vec, c(),
                      new_mat$alpha, size_point = 0,
                      str_c(name_stat, " en fonction des générations\nbottleneck ",
                            bott, " N0 ",size_pop," Nf ",size_pop),
                      TRUE,legd = TRUE, line_t = new_mat$U)
    
    p = improve_plot_bottle(p = p, vec_abline = c(1,bott+1), lab_col = "alpha",
                            lab_line = "U", vec_linetype = c("solid", "longdash", "dotted"))
    
    print(p)
  }
}

plot_without_point(mat_bottle, mat_bottle$Generation, 
              mat_bottle$mean.het.adm, mat_bottle$alpha,
              "Stat en fonction des générations\n selon différents bottleneck",
              legd = TRUE)

## `geom_smooth()` using method = 'gam' and formula 'y ~ s(x, bs = "cs")'

Moyenne de l’hétérozygotie

boucle_plot_bottle(mat_bottle, mat_bottle$mean.het.adm, "Heterozygotie", c(20,50,80))

Fst

boucle_plot_bottle(mat_bottle, mat_bottle$Fst.s1.adm, "Fst s1 adm", c(20,50,80))

boucle_plot_bottle(mat_bottle, mat_bottle$Fst.s2.adm, "Fst s2 adm", c(20,50,80))

boucle_plot_bottle(mat_bottle, mat_bottle$mean.F.adm, "F mean", c(20,50,80))

mini_mat_bottle = rbind(mat_bottle_time_20, mat_bottle_time_50, mat_bottle_time_80)

p = plot_stat_gen(mat_bottle, mat_bottle$Generation, 
              mat_bottle$mean.het.adm, c(),
              mat_bottle$bott_u, line_t = mat_bottle$alpha,
              "Stat en fonction des générations\n selon différents bottleneck",TRUE,
              legd = TRUE)
p = p+  theme(legend.position="bottom", legend.box = "horizontal")
print(p)

p = plot_stat_gen(mini_mat_bottle, mini_mat_bottle$Generation, 
                mini_mat_bottle$mean.het.adm, c(),
                mini_mat_bottle$bott_u, line_t = mini_mat_bottle$alpha,
                "Stat en fonction des générations\n selon différents bottleneck",TRUE,
                legd = TRUE)
improve_plot_bottle(p =p, vec_abline = c(21, 51, 81), lab_col = "tpsbott/U",
                    lab_line = "alpha", vec_linetype = c("solid", "longdash", "dotted"))

write_bottle_plot = function(name_stat, mat, name_file, min_y, max_y){
  num_col = which(colnames(mat) == name_stat)
  p = plot_stat_gen(mat, mat$Generation, 
                    mat[,num_col], c(),
                    mat$alpha_u, line_t = mat$time_botl,
                    str_c(name_stat, " en fonction des générations\n selon différents bottleneck\n (Ne0 = Nef = 1000)"),TRUE,
                    legd = TRUE)
  p = improve_plot_bottle(p =p, vec_abline = c(21, 51, 81), lab_col = "U/alpha",
                          lab_line = "tpsbott", vec_linetype = c("dotted", "longdash", "solid"))
  p = p+ylim(min_y, max_y)
  png(filename = str_c("../../../Images/",name_file,".png"), width = 1000, height = 800)
  print(p)
  dev.off()
  ggsave(filename =  str_c("../../../Images/", name_file, ".eps"), plot = p)
}

p = plot_stat_gen(mini_mat_bottle, mini_mat_bottle$Generation, 
                  mini_mat_bottle$mean.het.adm, c(),
                  mini_mat_bottle$alpha_u, line_t = mini_mat_bottle$time_botl,
                  "Moyenne He en fonction des générations\n selon différents bottleneck\n (Ne0 = Nef = 1000)",TRUE,
                  legd = TRUE)
p = improve_plot_bottle(p =p, vec_abline = c(21, 51, 81), lab_col = "U/alpha",
                        lab_line = "tpsbott", vec_linetype = c("dotted", "longdash", "solid"))
print(p)

p = plot_stat_gen(mini_mat_bottle, mini_mat_bottle$Generation, 
                  mini_mat_bottle$Fst.s1.adm, c(),
                  mini_mat_bottle$alpha_u, line_t = mini_mat_bottle$time_botl,
                  "Fst s1 adm en fonction des générations\n selon différents bottleneck\n (Ne0 = Nef = 1000)",TRUE,
                  legd = TRUE)
p = improve_plot_bottle(p =p, vec_abline = c(21, 51, 81), lab_col = "U/alpha",
                        lab_line = "tpsbott", vec_linetype = c("dotted", "longdash", "solid"))
print(p)

p = plot_stat_gen(mini_mat_bottle, mini_mat_bottle$Generation, 
                  mini_mat_bottle$mean.F.adm, c(),
                  mini_mat_bottle$alpha_u, line_t = mini_mat_bottle$time_botl,
                  "Moyenne F en fonction des générations\n selon différents bottleneck\n (Ne0 = Nef = 1000)",TRUE,
                  legd = TRUE)
p = improve_plot_bottle(p =p, vec_abline = c(21, 51, 81), lab_col = "U/alpha",
                        lab_line = "tpsbott", vec_linetype = c("dotted", "longdash", "solid"))
print(p)

# p = plot_stat_gen(mini_mat_bottle, mini_mat_bottle$Generation, 
#                   mini_mat_bottle$delta.adm.props, c(),
#                   mini_mat_bottle$alpha_u, line_t = mini_mat_bottle$time_botl,
#                   "delta props en fonction des générations\n selon différents bottleneck\n (Ne0 = Nef = 1000)",TRUE,
#                   legd = TRUE)
# p = improve_plot_bottle(p =p, vec_abline = c(21, 51, 81), lab_col = "U/alpha",
#                         lab_line = "tpsbott", vec_linetype = c("dotted", "longdash", "solid"))
# print(p)

Ajout delta mean et var des proportions d’admixture pour les effectifs efficaces constants.

mini_mat_bottle = delta.adm.props(mini_mat_bottle)

write_bottle_plot("mean.het.adm", mini_mat_bottle, "moyenne_heterozygotie/moy_He_bottleneck",0.01,0.062)

## Saving 7 x 5 in image

write_bottle_plot("Fst.s1.adm", mini_mat_bottle, "Fsts1adm/fst_s1_adm_bottleneck", 0, 0.5)

## Warning: Removed 1 rows containing missing values (geom_point).

## Warning: Removed 1 row(s) containing missing values (geom_path).

## Saving 7 x 5 in image

## Warning: Removed 1 rows containing missing values (geom_point).

## Warning: Removed 1 row(s) containing missing values (geom_path).

write_bottle_plot("Fst.s2.adm", mini_mat_bottle, "Fsts2adm/fst_s2_adm_bottleneck", 0, 0.5)

## Saving 7 x 5 in image

write_bottle_plot("mean.F.adm", mini_mat_bottle, "Fadm/f_adm_bottleneck", -0.03, 0.03)

## Saving 7 x 5 in image

write_bottle_plot("delta.mean.adm.props", mini_mat_bottle, "delta_mean_adm/delta_mean_bottleneck", -0.25, 0.25)

## Saving 7 x 5 in image

write_bottle_plot("delta.var.adm.props", mini_mat_bottle, "delta_var_adm/delta_var_bottleneck", -0.2, 0.05)

## Saving 7 x 5 in image

mini_mat_bottle$log.delta.var = log2(abs(mini_mat_bottle$delta.var.adm.props))
write_bottle_plot("log.delta.var", mini_mat_bottle, "delta_var_adm/log_delta_var_bottleneck", -15, 0)

## Warning: Removed 8 rows containing missing values (geom_point).

## Saving 7 x 5 in image

## Warning: Removed 8 rows containing missing values (geom_point).

vec_tmp = c()
vec_tmp[which(mat_bottle$alpha == 0.1)] = 2
vec_tmp[which(mat_bottle$alpha == 0.5)] = 3
vec_tmp[which(mat_bottle$alpha == 0.9)] = 4

scatter3D(x = mat_bottle$Generation, y = as.numeric(as.character(mat_bottle$time_botl)),
          z = mat_bottle$mean.het.adm, colvar = as.numeric(vec_tmp),
          ticktype = "detailed",bty = "b2",pch = 16,
          theta = 70, phi = 0, type = "s",cex = 0.3, cex.lab = 0.6, cex.axis = 0.5,
          xlab = "generation", ylab = "bottleneck", zlab = "He moyen",
          col = c("#1B9E77", "#D95F02", "#7570B3"),
          colkey = list(at = c(2.3, 3, 3.7),
                        addlines = TRUE, length = 1, width = 0.5,
                        labels = c("0.1", "0.5", "0.9")),
          clab = "alpha",
          main = "He moyenne en fonction des générations et\n du temps de bottleneck")

remove(vec_tmp)

setwd("../../../Images/")

seq_alpha = c(0.1, 0.5, 0.9)
mat_bottle_time_80_u_0.01 = mat_bottle_time_80[which(mat_bottle_time_80$U == 0.01),]

hist.gif.props.adm(mat = mat_bottle_time_80_u_0.01, select_stat = "alpha", select_seq = seq_alpha,
                   title = "adm.props/hist_bottle", title_leg = "alpha")

##  [1] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
## [16] TRUE TRUE TRUE TRUE TRUE

####Evolution de la proportion d’admixture dans la population

mat_bottle_time_80_u_0.01$delta.mean.adm.props = NA
mat_bottle_time_80_u_0.01$delta.var.adm.props = NA

var.adm = function(s1, gen){
  return( (s1*(1-s1))/(2**gen) )
}

for (i in 1:nrow(mat_bottle_time_80_u_0.01)) {
  if (mat_bottle_time_80_u_0.01$Generation[i] == 0) {
    ref_adm = mat_bottle_time_80_u_0.01$mean.adm.props[i]
    mat_bottle_time_80_u_0.01$delta.mean.adm.props[i] = 0
    mat_bottle_time_80_u_0.01$delta.var.adm.props[i] = 0
  }else{
    mat_bottle_time_80_u_0.01$delta.mean.adm.props[i] = mat_bottle_time_80_u_0.01$mean.adm.props[i] - ref_adm
    ref_var = var.adm(s1 = ref_adm, gen = mat_bottle_time_80_u_0.01$Generation[i])
    mat_bottle_time_80_u_0.01$delta.var.adm.props[i] = mat_bottle_time_80_u_0.01$var.adm.props[i] - ref_var
  }
}

# plot(mat_bottle_time_80_u_0.01$delta.mean.adm.props~mat_bottle_time_80_u_0.01$Generation, type = "l")
# plot(mat_bottle_time_80_u_0.01$delta.var.adm.props~mat_bottle_time_80_u_0.01$Generation, type = "l")


plot_stat_gen(mat_bottle_time_80_u_0.01, mat_bottle_time_80_u_0.01$Generation, 
              mat_bottle_time_80_u_0.01$delta.mean.adm.props,
              mat_bottle_time_80_u_0.01$alpha, mat_bottle_time_80_u_0.01$alpha,ligne = T,
              "Delta mean adm props en fonction des générations\n bottleneck = 80, U = 0.01",
              legd = TRUE)

plot_stat_gen(mat_bottle_time_80_u_0.01, mat_bottle_time_80_u_0.01$Generation, 
              mat_bottle_time_80_u_0.01$delta.var.adm.props,
              mat_bottle_time_80_u_0.01$alpha, mat_bottle_time_80_u_0.01$alpha,ligne = T,
              "Delta var adm props en fonction des générations\n bottleneck = 80, U = 0.01",
              legd = TRUE)

# vec_test_tot = c()
# level = "0.1"
# for (level in levels(mat_bottle_time_80_u_0.01$alpha)) {
#   mat_tmp = mat_bottle_time_80_u_0.01[which(mat_bottle_time_80_u_0.01$alpha == level),]
#   vec_test = c()
#   for (gen in 1:102) {
#     vec_test[gen] = (1 - mat_tmp$mean.adm.props[gen])
#   }
#   if (level == levels(mat_bottle_time_80_u_0.01$alpha)[1]) {
#     vec_test_tot = cbind(vec_test, rep(level, length(vec_test)), seq(0,101,1))
#   }else{
#     vec_test = cbind(vec_test, rep(level, length(vec_test)), seq(0,101,1))
#     vec_test_tot = rbind(vec_test_tot, vec_test)
#   }
# }
# vec_test_tot[, 1] = as.double(vec_test_tot[, 1])
# colnames(vec_test_tot) = c("stat", "simu", "gen")
# vec_test_tot = as.data.frame(vec_test_tot)
# p = ggplot(vec_test_tot, aes(x = gen, y = stat,group = simu))
# 
# p = p + geom_line()
# p = p + theme_classic()
# print(p)

###Bottleneck 1000

print(difftime(Sys.time(), time.ini))

## Time difference of 4.848785 mins

####N0 = Nf = 10.000

setwd("../new_methis_bottleneck_50000_snp/")

mat_bottle_10.000 = data.frame.bottleneck(lst_ne0 = c(10000), lst_nef = c(10000),
                                           lst_alpha = c(0.01, 0.1, 0.5, 0.9),
                                           lst_nu = c(0.01, 0.25, 0.49),
                                           lst_bottle = seq(10,90,10))

mat_bottle_time_50_10.000 = mat_bottle_10.000[which(mat_bottle_10.000$time_botl == 50),]
mat_bottle_time_20_10.000 = mat_bottle_10.000[which(mat_bottle_10.000$time_botl == 20),]
mat_bottle_time_80_10.000 = mat_bottle_10.000[which(mat_bottle_10.000$time_botl == 80),]

Moyenne de l’hétérozygotie

boucle_plot_bottle(mat_bottle_10.000, mat_bottle_10.000$mean.het.adm, "Heterozygotie", c(20,50,80),10000)

mini_mat_bottle_10.000 = rbind(mat_bottle_time_20_10.000, mat_bottle_time_50_10.000, mat_bottle_time_80_10.000)

p = plot_stat_gen(mini_mat_bottle_10.000, mini_mat_bottle_10.000$Generation, 
                  mini_mat_bottle_10.000$mean.het.adm, c(),
                  mini_mat_bottle_10.000$alpha_u, line_t = mini_mat_bottle_10.000$time_botl,
                  "Moyenne He en fonction des générations\n selon différents bottleneck\n (Ne0 = Nef = 10000)",TRUE,
                  legd = TRUE)
p = improve_plot_bottle(p =p, vec_abline = c(21, 51, 81), lab_col = "U/alpha",
                        lab_line = "tpsbott", vec_linetype = c("dotted", "longdash", "solid"))
print(p)

###Bottleneck 10000

print(difftime(Sys.time(), time.ini))

## Time difference of 4.983517 mins

####N0 = Nf = 100.000

setwd("../new_methis_bottleneck_50000_snp")
options( "scipen"=100) 
#/alpha0.1/Nu0.01/bottle10/Ne100000-XXX/Ne100000-100000/simu_1/

mat_bottle_100.000 = data.frame.bottleneck(lst_ne0 = c(100000), lst_nef = c(100000),
                                           lst_alpha = c(0.001, 0.01, 0.1, 0.5, 0.9),
                                           lst_nu = c(0.01, 0.25, 0.49),
                                           lst_bottle = seq(10,90,10))
#seq(10, 90, 10)

# mat_bottle_time_50_100.000 = mat_bottle_100.000[which(mat_bottle_100.000$time_botl == 50),]
# mat_bottle_time_20_100.000 = mat_bottle_100.000[which(mat_bottle_100.000$time_botl == 20),]
# mat_bottle_time_80_100.000 = mat_bottle_100.000[which(mat_bottle_100.000$time_botl == 80),]

options( "scipen"=0) 

boucle_plot_bottle(mat_bottle_100.000, mat_bottle_100.000$mean.het.adm, "Heterozygotie", c(20,50,80),"100 000")

###Bottleneck 100 000

print(difftime(Sys.time(), time.ini))

## Time difference of 5.127488 mins